place in the last 100 years (Preece, 2003). A, different growth regulators and types of cuttings. Environmental conditions can affect the, ability of cuttings to grow and induce root. The asexual methods for propagation, are used to clone chosen genotypes of breeding methods and to establish commercial fruit, orchards, as they provide all the distinctiveness of each cultivar, various methods used in the propagation of guava, which are commercially adopted, and the, recent progress and trends related to it. The environmental composition plays a, crucial role in the morphogenesis of plant. Fracaro, 2004 found, the primary roots had, sufcientnumbers,adistributedaroundthe, suitable growth and high branching, providing, exploration of large volumes of soil in search of, early beginning of the root and on the formation, layering is commonly adopted viz Air layering and. The guava plants can be propagated through seed, inarching, layering, cutting, budding and grafting. and hydrochloric acid (78%) on control (20%). Planting seeds Guava seeds are usually started in nursery beds or pots before being transplanted in the field or garden. and pulmonary disease in Bolivia and Egypt (Batick, 1984). The roots are induced within 2-3 months, depending on climatic conditions. oportoalegre:vecontinents.cap.;5:85-111. Maximum number of explants (76.6%) responded to rooting with 1.5 mgl-1 IAA in combination with 1.0 mgl-1 NAA. Guava is known for its tolerance to salt, whereas it is sensitive to water logging condition. The study was undertaken with the objective of exploring the technique of Air and stool layering in guava propagation with the aid of IBA and to determine the optimum time of layering and rooting media. The latest information has revealed, ., 2004). Singh et al. Spraying the, appropriate fungicides 3-4 days before transferring, the plants to a greenhouse also reduces the risk, of fungal contamination of explants. September to March is, the appropriate time for the preparation of cuttings, (southern hemisphere), when the plants are in full, growth. percentage of germination was recorded with conc. through cutting under controlled conditions. © 2008-2020 ResearchGate GmbH. D.B. best performance (73.3 to 83.3% success rooting, 5.82 to 7.16 primary roots and 101.2 mg to, 112.4mg dry weight of roots) regardless of the, parameters (Debnath and Maiti 1990). Stem nodes from these lateral shoots were Although guava is hard-to-root, investigations have indicated that it can be successfully propagated from cuttings under mist. The diameter of the maximum average stem, the. It is commonly called as guava, alteration for stress tolerance, genomics use, molecular physiology and bioinformatics can be, used to understand plant physiology in plants better, with changing weather conditions and development, sources has been hampered by higher microbial, contamination and exudation of the phenols at, higher level from plant tissues will resulting in the, removal of the darkness and recalcitrant nature of, plant tissues. Vegetative propagation of guava through softwood cuttings is excellent for nursery plants production because it is cheap, fast and economical clonal propagation method [10]. Irrespective of cutting type, IAA and NAA seem to be the most efficient in promoting roots. B. have been hampered due to juvenile phase of longer, duration, season reliance, long life span, and, increased plant propagation material (Jaiswal and, air layering are also used as a multiplication method, in various countries. The only way out, is the varietal development those are resistant to, is a major concern. “Lucknow-49” has been treated in, surface area ringed with various concentrations, vermin-compost and FYM. Psidium guajava L. through cuttings. When it comes to propagating guava trees with seeds, the best plan is to plant fresh seeds from a ripe, juicy fruit. experiment. However, these, methods have also been analyzed, as well as of an, entire plant development by embryogenesis and, facilitates the replication of elite genotypic plants, for later evaluation and also provides orchard, grower with disease-free vegetative propagation, the effect of treatment and subculturing on shoot, R.M.Radicaldisinfestationprotocoleliminates, propagation of guava through in vitro shoot. Whereas on MS containing glutamin 250 mgl-1, 25% explants responded and gave 2.2 shoots per explants after 46-days. For grafting purposes, the seedlings should, improved by immersing it in tap water before, but immersion in hot water had been detrimental, stated that dipping the guava seeds in distilled, water for 48 hours had 96% germination. embryos, while the addition of glucose, fructose, maltose, sorbitol and mannitol in the medium, a better initiation of embryos as compared to the, liquid medium. Objetivo. Maximum survival, number of shoots in polyhouse, regardless of the, possible IBA concentrations that provides good, environmental conditions seedlings compared, to uncontrolled environmental conditions open, developed from the buds of green shoots in guava. As compared to control, auxin treatment promoted rooting in both types of cuttings. Varieties are rather difficult to propagate by the … The propagation of the guava (Psidium guajava L.) may be carried out with seeds, layering, grafting or budding, cuttings (roots or shoots) or by micropropagation. Australian Journal of Crop Science, Lismore. Patch, budding was more popular method to propagate, for Allahabad Safeda among budding (Bhatt, guava transplantation. budding from August 15th to 21st showed the, highest success rate (92.07%) observed after, reported that the patch budding mid-June showed. In vitro propagation of guava (Psidium guajava L.) shoots by direct organogenesis is an alternative to quickly obtain productive plants. Nevertheless, a few species may sprout, throughout winter when they are inactive. The The highest number of roots (23.75) per cutting was, recorded in the cutting treated with IBA at 4000, was observed in the stem treated with IAA 3000, ppm. propagation of guava species by stool layering. AVANÇOS NA PROPAGAÇÃO DA GOIABEIRA RESUMO-A goiabeira (Psidium guajava L.) pode ser propagada por sementes, mergulhia, alporquia, en-xertia (borbulhia ou garfagem), estaquia (de raiz ou de ramos) ou pela cultura de tecidos. Comparatively IAA was stronger than IBA in sprouting for both types of cuttings. Singh, with rooting media helped produce the maximum, number of primary roots, secondary roots, leaves, in 60 days and length of shoots in 60 days. El cultivo de guayaba en Colombia presenta rendimiento inadecuado en las diferentes regiones productoras, debido principalmente al desconocimiento por parte de los productores y al uso de tecnologías que no son adecuadas para la producción intensiva. Partial shading is necessary until the seedlings have established. A preview of the PDF is not available. important fruit crops in agroforestry system. The rootstock stem is separated between 15 to. However, when the concentration of glutamin alone was increased from 250 to 500 mgl-1 the shoot formation potential of explant decreased to 10%. The most suitable period for conducting, girdled area must be selected. Remove the seeds from the pulp and wash them thoroughly. Once 50% of the, roots are induced, the layers are removed from the, parent plant and stored in a 5 liter polyethylene bag, until they become strong enough to be transplanted. This is the simplest technique for the, propagation of guava trees. In case of budding, the patch budding performed during 15 to 21 August showed highest per cent success (92.07%) recorded after 90 days of guava propagation. The asexual methods for propagation are used to clone chosen genotypes of breeding methods and to establish commercial fruit orchards, as they provide all the distinctiveness of each cultivar. can vary between 70 and 92%.” (Singh, 2007). commercial use are normally considered waste. using leaf disc as explant in guava cultivars. Somatic, cells produce masses that develop into mature, embryos under favorable growth conditions in. and quality (Dhua and Sen, 1984). of shooting percent, roots number per cutting, root cutting in colder regions such as California, was the main advantage, as well as the prospect, of propagating guava plants by cuttings. nitrogen actually induced somatic embryogenesis, while PEG and L-proline accelerated the maturity, the different sources of carbon, 5-6% sucrose, was better for inducing and maturing of somatic. Choice varieties can be increased only by some vegetative means of propagation. cuttings and have young leaves that grow actively. Malavolta E. 2000. However, guava hardwood cuttings were found hard to root (Luis et al., 1986) [33]. (2007) [53, 68, 81, 82] observed that the combination of IBA with rooting media helped in producing maximum number of primary roots, secondary roots, leaves on 60 days and length of shoots on 60 days. cultivation in Guava, if propagated through seed, exhibits a great variation due to inevitable heterogeneity. Propagation: Raising of commercial orchards through seed is not advisable. indica: a tropical medicinal tree species. Other aspects of guava cultivation. after union, to avoid dehydration and to boost the, success rate. safeda seedling explants, Propagation of citrus species through cutting: A review, Study the Effect of Different Growing Conditions and Varieties on Graft-Take, Leaves and Scion Diameter in Guava (Psidium guajava L.), Effect of Grafting Time on Growth and Success Rate of Guava (Psidium guajava L.) Wedge Grafts Grown under Shade Net and Poly House Conditions, Vegetative Propagation of Pomegranate (Punica granatum L.) through Cutting- A Review, Studies on Performance of Softwood Grafting in Guava (Psidium guajava L.) cv. most common external factors are light, seasons, temperature, humidity, moisture level of the cutting. Introducción. increased the number of leaves (16) per cutting. Maximum success percentage (59.26) was achieved in plants budded by T-grafting followed by T-budding (22.58%). On MS containing combination of BA (2.0 mgl-1) and zeatin (1.0 mgl-1), 37% explants responded which produced 2.3 shoot per explant after 42 days of culturing. Air-layering was evaluated as a commercial method of vegetative propagation of guava. The disadvantage is. UniversidadeFederaldePelotas,Pelotas;66. species independently of hormonal combinations. plants with essential characteristics is required. In case of grafting, wedge grafting in the month of August gave better results in polyhouse (69.88 %) as well as in open field condition (67.12 %). Eds. At alternate, seeds will germinate in the darkness, suggesting, that in the cracks of the foliage, when the seeds are, covered by a thin layer of soil, will sprout once the, alternating temperatures are experienced. Using vegeta - tive propagation, a large number of nursery plants with the same genetic makeup can be obtained at one time, Florence. Cuttings must have three internodes and four nodes. of energy crisis. growth of stooled planlets. There are several propagation technologies available, however, the adoption levels are rather different between producing countries. Explants taken from 49-day old seedlings were cultured on MS-medium modified with different concentration of plant growth regulators. During winter cultivation early fruit production correlated well with vegetative growth induced by the rootstock, but this correlation was almost absent during cultivation in the summer. Serial minicutting technique for guava 'Paluma' propagation.pdf. Introduction Guava (Psidium guajava L.) belong to family Myrtaceae. in vitro can facilitate rapid clonal propagation. By contrast, IBA was more effective only in soft wood cuttings. How to Propagate Guava. The various propagation techniques are available, but the levels of adoption are quite different in guava producing areas. But air-layering along with exogenous application of auxin has stimulating effect on producing longer roots, remains the best method of propagation for this crop. During winter cultivation the rootstocks cultivars ‘Renova’ and ‘Esvier’ significantly promoted vegetative growth of the scion and rootstock Code E induced a significantly higher fruit yield than the standard rootstock ‘Corona’. non volatile organic compound of guava fruit includes citric, maleic, lactic, ascorbic and increased sensitivity to abiotic stress such as frost, biology such as marker-based selection, genetic. and sometimes in guava also. 3. Organogenesis was found in Banarasi, Safeda, Allahabad Safeda and Beaumont varieties, was more effective than explants of mature, woody plants and explants of young greenhouse-, also found that though, in the cultivars Beaumont, and Allahabad Safeda shoots regeneration was, established by explants from nodes of mature, plants. were the second best for the survival of air layers. embryogenesis helps to study plant differentiation, totipotent cell expression level also and has been, Safeda and Banarasi cultivars, using the zygotic, embryos as explants. Maximum explants showing shoot response (48%) was observed on MS-medium supplemented with 6-benzyladanin (BA) 1.0 mgl-1combined with 0.5 mgl-1ziatin. cm size are best suited for in vitro propagation of guava (Amin and Jaiswal, 1987, 1988; Loh and Rao, 1989; Siddiqui and Farroa, 1996, Meghwal et al., 2003; Bisen, 2004; Zamir et al., 2007, Xiaomei and Yang, 201 1). By using chemicals, presently, there are no control measures. “the poor man’ s fruit” or “apple of tropics”. The seed propagation is now restricted to raising of rootstock materials. Further experiments involved 21 and nine rootstock accessions, respectively, and scion genotype cultivar ‘Corona’. The chosen, shoot should be cut and defoliated on the parent, plant between 5 and 7 days before separation. After leaving the time necessary to start the root, rooted shoots are separated and taken as individual, layers. This, can be particularly important if the wilt-resistant, method has become popular. Macro-propagation of Guava (Psidium guajava L.) 1Mir Saleem Khattak, M.N. main reasons for rooting in cuttings are plant age, used commercially. Sin embargo, actualmente existe una serie de ofertas tecnológicas que pueden ser implementadas para abordar las principales limitantes del cultivo, logrando como resultado mejorar la producción de guayaba de manera rentable y ambientalmente sostenible. It was reported that guava is commercially propagated from seeds in Pakistan9. the stock and scion are placed in close contact with each other and held together firmly, until they unite to form a composite plant. Necessidades para melhorias na formação de mudas de goiabeira são discutidas. Then the, seeds are extracted, washed with running water, and dried in the shade for 10 days. All shoots growing under the graft, should be removed regularly to avoid competition, developed root system of the rootstock. This review article deals with various methods used in the propagation of guava, which are commercially adopted, and the recent progress and trends related to it. The study describes the scope of clonal propagation of guava (Psidium guajava Linn.) The growth of these layers was vigorous, as. However, combination of BA with zeatin was effective in shooting response, number of shoot/explants and shoot length. It is now known that, treatment with etiolation increases the temporary, accumulation of endogenous growth substances, in the etiolate portion due to some anatomical, abnormalities, which promote better root rooting. J.2009. Conclusiones. For air layering the operation was done in 3 months i.e. Cutting with root promoters, (synthetic auxines such as indolic butyric acid, (IBA) or naphthalene acetic acid (NAA)) treatments, and stored in pot in mist diffusion system, with a, normal water jet (intervals of 5 seconds at every, 5 minutes). Safeda. Training manual agroforestry technologies for, Lutfullah, G. In vitro regeneration of plantlets, Jagadeesh SL. Detection of growth, regulators, information on role of the juvenile, stage in reproduction, advances in knowledge of, chimeras, micro-propagation and application of, vegetative propagation to prevent diseases caused, by viruses and other pathogens (Preece, 2003, 2001), stoolings (Pathak and Saroj, 1988) and, inarching (Mukherjee and Majumder, 1983) are not, yet commercially feasible due to different success, rates, the lack of a tap root system and burdensome, processes. into the garden.” (Hartman and Kester, 2002). “T” budding or shield budding is the, most common budding method in guava. Place of origin of guava is tropical America. new technologies must also be taken into account. 3-Butyric acid, the cuttings had not induced root. a better response to the number of sprouting (7.49), percentage of survival (73.33%), average length of, the shoot (50.27 cm), average leaf length in new, growth (6.67 cm), average leaf width (3.71 cm), out instantaneously prior to or during the growth, period. the use of seeds in plants has great variability. A presente revisão bibliográfica tem por objetivo abordar os diferentes métodos que podem ser utilizados para propagar a goiabeira, os comercialmente adotados e os avanços obtidos nos últimos anos. Because, of the intense cold that caused the freezing and, death of trees in orchards, it was likely to use new, shoots from the root system to propagate new, plant without a graft because the root system is, genetically identical to the stem. But air-layering along with exogenous application of auxin has stimulating effect on producing longer roots, remains the best method of propagation for this crop. species of trees and shrubs, many of which have edible fruits. Initially surface sterilized guava seeds were germinated inside the conical flasks. fruit is an excellent source of vitamin-C (Rathore, 1976; Yadava, 1994), which is upto 2000 by stem cutting collected from mature stockplants. This is considered. It is, important to know how to use the same methods, of graftings and cutting. proliferation on nodal explants of mature trees. It is well-distributed in. First, the soil fungus caused guava wilt disease to, a damaging effect. Sardar as Influenced by Different Shade Intensity, Effect of Different Auxins on Rooting of Semi Hard and Soft Wood Cuttings of Guava (Psidium guajava L.) CV. The most common external factors are light, seasons, temperature, humidity, moisture level of cutting and rooting medium (Hartman et al., 1997) [22]. galacturonic acid (Chan et al., 1971). In the case of shoots removed, seedlings established in vitro, the roots were grown, gel substnaces, agar has been widely used during, organogenesis in various cultivars and there is no, means for plant biotechnology and is broadly used, for mass propagation of best cultivars. among young plants (Paull and Bittenbender, grafting technology, the seed is used for the, cultivation of rhizomes. Na produção de porta-enxertos e na fase inicial do melhoramento genético as guava the. Progress obtained in recent years seeds may, be sown in nurseries or directly in polybags which... Be done for propagation systems, in particular, there are several propagation technologies,... Ready for field transplanting or used as rootstocks when they are six months to one old... Of cutting type, IAA and NAA seem to be second best for the, cultivation rhizomes... Developed root system of the world fungus caused guava wilt disease to, produce basal leaves about 10mm above root. Knowledge from anywhere country occupies 1,62,000 hectare land ( NHB, Indian horticulture database )... Addition, the soil successfully main reasons for rooting in both types of cuttings AI-powered research for!, ( Pereira, 1990 ) determinar el estado actual de la guayaba en Colombia Haq et al., )... For wedge grafting in guava graft, should be made with 3-4 buds, 15-18cm long 8mm... The cambium layer between bark and, wood must be taken that the propagation guava! In Bolivia and Egypt ( Batick, 1984 ) the country occupies 1,62,000 hectare (. 78 % ) responded to rooting with 1.5 mgl-1 IAA in combination with 1.0 mgl-1 NAA about 6 to hard... Shield, patch, chip, etc is commercially propagated from seeds in water! These lateral shoots ( 3.4 vs 1.2 per seedling ) be propagated asexually by root cuttings, stored! Pereira, 1990 ) indexação: Psidium guajava L. ) belong to family Myrtaceae in water! Boost the, most common guava cultivated and is highly prized throughout the tropics and warm sub-tropics of cutting... The leaf, can be particularly important if the wilt-resistant, method has become.. Was found to be the most efficient in promoting roots ( Hartman and,! Of plant months viz highest success rates in, the seedlings have established 1984 ) led to differences in and... Treatments on rooting of guava seed was improved by soaking it in tap water before sowing recommended. Drying for 2. days to learn more about guava cutting propagation and growing guava trees 4 months, mid-June. Compounds ( Mortan, 1987 ) early phases in the field or garden. 0, 0.2 % 0.4. With zeatin was effective in shooting response, number of roots ( 27.6 ) per cutting was recorded GA3! Experts in, surface area ringed with various concentrations, vermin-compost and FYM and scion are, compatible propagated with. Key in such studies the operation was done in 3 months i.e (... Poor man ’ s fruit ” or “ apple of tropics ” own! Of cutting type, IAA and NAA were greater than semi hard wood cuttings by roots of vegetative. “ Lucknow-49 ” has been treated in, the seedlings have a root system... Water logging condition ResearchGate to discover and stay up-to-date with the latest information has,. Supplemented with 6-benzyladanin ( BA ) 1.0 mgl-1combined with 0.5 mgl-1ziatin selection, genetic investigations have indicated it... Commercially propagated from cuttings under mist only way out, is removed regulator like has., number of shoot/explants and shoot propagation of guava pdf been tried, such as marker-based,. Absorption system with this, exercise helps the swollen buds that can sprout, throughout winter when they are months! Be grouped into 2 groups – Red flash types: Red fleshed fruit contains mg. Non-Mist propagator was vigorous, as cultured on MS-medium modified with different concentration of plant produce basal leaves 10mm. Commercial, nurseries, respectively, and scion are, compatible explants taken 49-day... ( 2015 ) and wash them thoroughly fruit is attributed to carbonyl compounds ( Mortan, 1987.. If propagated through seed, inarching, layering, grafting technology, the seed propagation is free... Process of asexually reproducing propagation of guava pdf by multiplication of one copy to produce several copies that are genetically identical addresses methods... By root cuttings, are stored on a steam-sterilized root substrate to,.. About 6 to 8 hard leaf buds, 15-18cm long and 8mm in diameter various concentrations vermin-compost! Conducted to find out best month for wedge grafting in guava out of four viz... Of roots ( 27.6 ) per cutting was recorded in soft wood cuttings treated with,! Wedge grafting in guava producing areas and generally occurs during 15-20, days however, the highest percentage! Of carotene/100 g of talc. ”, IBA ( 4000ppm ) showed maximum results low! Storage aroma of fruit had not propagation of guava pdf root poultry manure healthy, vigorous trees … etc roots. For fresh fruit are usually vegetatively propagated by air layering on guava cv to and. With different concentration of plant seed was improved by soaking it in tap before! Mg of carotene/100 g of fruit best methods of vegetative propagation of different guava varieties be... Discover and stay up-to-date with the latest research from leading experts in, addition the. Variability in root induction the purpose of the production, of graftings and.. Propagation has assured true-to-type plants ) recommended the manual collection of,., 2004 ) of,. Be cut and defoliated on the production chain logging condition in cuttings treated with IBA symptoms. Tolerance to salt, whereas it is commonly called as guava, the success is dependent on early initiation! 48 % ) on control ( 20 % ) may, be sown in nurseries or directly in month! Mgl-1 NAA, these techniques, can ’ t have Access to guava! Comes from the soil surface and grafting germination in guava cuttings under mist,.... Family Myrtaceae the, cultivation of rhizomes of seed germination, the leaf, can ’ t be used the... From these lateral shoots were Partial shading is necessary until the seedlings have established of vegetative propagation of guava will... Series of external, and internal factors a steam-sterilized root substrate to, a maximum,! Trees will be discussed experiments involved 21 and nine rootstock accessions, respectively, and are! ) can be used to treat many ailments including cough and pulmonary disease in Bolivia Egypt. Tree, you can purchase a guava tree, you can purchase a guava tree, you can a! Glutamin 250 mgl-1, 25 % explants responded and gave 2.2 shoots per explants after 46-days “ the man! Long was also recorded stem branches is used, guava hardwood cuttings were found hard to root Luis... The root, rooted shoots are separated and taken as individual, layers then the, common. The wilt-resistant, method has become popular polyethylene, is removed, plant did not have,! Ms-Medium modified with different concentration of plant growth regulators 95 % of success, but more as! And shifted to soil successfully recommended, only in breeding programs contrast, IBA 4000ppm! After union, to avoid competition, developed root system of the rootstock in different fruit propagation of guava pdf the second for..., 1990 ) once the transplant is complete 90 % and 0.8 % IBA and... Damaging effect to, fungus external factors are light, seasons, temperature, humidity, moisture of. Saleem Khattak, M.N for, Lutfullah, G. in vitro propagation guava... For survival of plants is the most popular commercial method of propagation of. Plant survival is, however, these techniques, can be used for large scale because the.! Iba was more effective only in soft wood cuttings soft wood cuttings, are... Peace vegetative propagation ( Hartman, 1969 ) [ 21 ] and the of. Propagation of different guava varieties can be successfully propagated from cuttings of in. Grafting and inarching overcome difficulties in root induction rootstocks when they are inactive a, longer juvenile,. The world it in tap water before sowing induced within 2-3 months, depending on climatic.. This manual was produced by roots of Peace under USAID subcontract no extracted. Is as easy as harvesting and planting the seeds may, be sown in nurseries or directly in polybags which... ( 27.6 ) per cutting type, IAA and NAA were greater than semi hard wood cuttings treated, IAA. System of the cutting guava, “ the shoot should be treated before use against due! Uide F_N-49 July 201 is no root problem with, this technique can, exceed %... Or “ apple of tropics ” must be selected and Skoog ( MS ) with... Commercial method of propagation to overcome difficulties in root induction to 95 % of success, but more as! Os países produtores guava trees from cuttings under mist waytocloneguavaplantsand, could be the common! ’ t have Access to a guava at a grocery store or farmers market:... The progress obtained in recent years to treat many ailments including cough and pulmonary disease in Bolivia and (!, could be the most suitable period for conducting, girdled area must be taken the! Of guava-wilt resistant, rootstocks, this method, the methods commercially adopted and the polyethylene, is the cultivation. Warm sub-tropics of the production of the site may not work correctly irrespective of cutting,. For vegetative propagation ( Hartman and Kester, 2002 ) is necessary until the seedlings have root! Defoliated on the parent, plant did not have a, different growth and. Access to a guava tree, you can purchase a guava tree porta-enxertos e na fase inicial do genético... Key to such studies more about guava cutting propagation and growing guava trees will be discussed rooting in treated! Plants that the study describes the scope of clonal propagation has assured true-to-type plants months! To the propagation structure, the seed strength the simplest technique for the, ability stem.

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